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	Provedor de dados BABT (1) BJMBR (1) Autor Dolder,Mary Anne Heidi (1) Genari,Selma Candelária (1) Oliveira,Naila Francis Paulo de (1) Palavra-chave Percoll (2) Blood (1) Germ cell separation (1) In vitro (1) Lymphocytes (1) Microscopy (1) Mitochondrial cytochrome C oxidase II (COX II) (1) Molecular analysis (1) Morphology (1) Rat testis (1) Sulfated glycoprotein 1 (SGP1) (1) Transition protein 2 (TP2) (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Info:eu-repo/semantics/other (1) Ano 2010 (1) 1997 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Assessment of the degree of contamination of rat germ cell preparations using specific cDNA probes BJMBR Recent reports showing a decrease in sperm count in men have brought new concerns about male infertility. Animal models have been widely used to provide some relevant information about the human male gamete, and extrapolations are made to men and to the clinical context. The present study assesses one of the methods used for separation of germ cells of the adult rat testis, namely centrifugal elutriation followed by density gradients (Percoll®). This method was chosen since it presents the best results for cell purity in separating germ cells from the rat testis. A comparison between continuous and discontinuous Percoll® gradients was performed in order to identify the best type of gradient to separate the cells. Maximal cell purity was obtained for... Tipo: Info:eu-repo/semantics/other Palavras-chave: Rat testis; Germ cell separation; Percoll; Molecular analysis; Transition protein 2 (TP2); Mitochondrial cytochrome C oxidase II (COX II); Sulfated glycoprotein 1 (SGP1). Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300007 Light microscope observation of circulating human lymphocytes cultured in vitro BABT Oliveira,Naila Francis Paulo de; Dolder,Mary Anne Heidi; Genari,Selma Candelária. The purpose of this work was to study the isolation and a light microscopy technique for cultured lymphocytes. Blood samples were obtained by venipuncture with an anticoagulant added and centrifuged in a Percoll density gradient to separate the leukocytes. Lymphocytes were placed in 25 cm ³ tissue culture flasks at 37ºC. After culturing, they were fixed and stained with the methods used for blood smears. Results showed that not all fixing solutions and stains were an equally good choice for cultured lymphocytes. Tipo: Info:eu-repo/semantics/article Palavras-chave: Blood; In vitro; Lymphocytes; Microscopy; Morphology; Percoll. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500013 Registros recuperados: 2 Primeira ... 1 ... Última

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